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- Title
Comparing 16S rDNA amplicon sequencing and hybridization capture for pea aphid microbiota diversity analysis.
- Authors
Cariou, Marie; Ribière, Céline; Morlière, Stéphanie; Gauthier, Jean-Pierre; Simon, Jean-Christophe; Peyret, Pierre; Charlat, Sylvain
- Abstract
Objective: Targeted sequencing of 16S rDNA amplicons is routinely used for microbial community profiling but this method suffers several limitations such as bias affinity of universal primers and short read size. Gene capture by hybridization represents a promising alternative. Here we used a metagenomic extract from the pea aphid <italic>Acyrthosiphon pisum</italic> to compare the performances of two widely used PCR primer pairs with DNA capture, based on solution hybrid selection. Results: All methods produced an exhaustive description of the 8 bacterial taxa known to be present in this sample. In addition, the methods yielded similar quantitative results, with the number of reads strongly correlating with quantitative PCR controls. Both methods can thus be considered as qualitatively and quantitatively robust on such a sample with low microbial complexity.
- Subjects
RECOMBINANT DNA; PEA aphid; NUCLEIC acid hybridization; PHYLOGENY; MICROBIAL ecology
- Publication
BMC Research Notes, 2018, Vol 11, Issue 1, pN.PAG
- ISSN
1756-0500
- Publication type
Article
- DOI
10.1186/s13104-018-3559-3