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- Title
Determination of Cytotoxicity of Latex and Methanol Extract of Euphorbia helioscopia Leaves on Vero Cell Line with MTT Assay.
- Authors
Ahmad, Bashir; Ahmad, Mobasher; Hussain, Khalid; Bukhari, Nadeem Irfan; Saleem, Uzma; Anjum, Aftab Ahmad
- Abstract
The objective of this study was to evaluate the cytotoxic potential of latex and leaves methanol extract of Euphorbia helioscopia on Vero cell line. Aliquots (100 µl) of eight different concentrations (1000-0.0128 µg/ml) of each sample were incubated (with 5%CO2) with 100 µl of 0.5% MTT solution for 48 h at 37°C. Active mitochondria of viable cells cleaved tetrazolium ring of MTT and consequently purple colored formazan was formed. Then multi-well plate was read in ELIZA reader at 570nm. Latex showed 57% cell survival percentage at highest (1000 µg/ml) while leaves methanol had 34% cell survival percentage at same concentration. IC50 of leaves methanol extract, calculated from linear regression equation, was 5.27 µg/ml. It was concluded that latex was devoid of cytotoxicity within concentrations ranged from 1000 to 0.0128 µg/ml, whereas leaves methanol extract showed cytotoxicity at 5.27 µg/ml concentration.
- Subjects
VIABILITY (Biology); EUPHORBIA; LATEX; PLANT extracts; METHANOL
- Publication
Pakistan Journal of Zoology, 2014, Vol 46, Issue 3, p741
- ISSN
0030-9923
- Publication type
Article