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- Title
Anti-adipogenic effects of Tropaeolum majus (nasturtium) ethanol extract on 3T3-L1 cells.
- Authors
Kim, Gi-Chang; Kim, Jin-Sook; Kim, Gyoung-Mi; Choi, Song-Yi
- Abstract
Background: Edible flowers, Tropaeolum majus has been used as a disinfectant and an antibiotic, and for wound healing, but the anti-obesity effects of this plant have not been reported previously Objective: We investigated the anti-adipogenic effects of T. majus ethanol extract (TME) on 3T3-L1 cells. Design: 3T3-L1 cells were differentiated in the presence of different concentrations of TME. Lipid accumulation levels were determined using Oil-Red O staining and a triglyceride content assay. Changes in the expression of proteins related to adipocyte differentiation in 3T3-L1 cells were determined by SDS-PAGE and western blotting. Results: The highest inhibition of lipid accumulation was observed at a TME concentration of 300 µg/mL. Additionally, TME concentrations ranging from 20 µg/mL to 500 µg/mL led to a decrease in the expression of adipocyte differentiation regulators, peroxisome proliferator-activated receptor γ, CCAAT element binding protein α, and sterol regulatory element binding transcription factor 1. This decrease was shown to be concentration-dependent. Discussion: Taken together, the results of this study demonstrate that TME inhibits lipid accumulation and reduces the expression PPARG, CEBPA, and SREBF1, which regulate adipocyte differentiation in 3T3-L1 cells. Conclusions: TME may be a potential novel therapeutic agent for the prevention and treatment of obesity.
- Subjects
CELL differentiation; CELL lines; ETHANOL; FAT cells; GENE expression; OBESITY; PLANTS; TRANSCRIPTION factors; TRIGLYCERIDES; WESTERN immunoblotting; PEROXISOME proliferator-activated receptors
- Publication
Food & Nutrition Research, 2017, Vol 61, Issue 1, pN.PAG
- ISSN
1654-6628
- Publication type
Article
- DOI
10.1080/16546628.2017.1339555