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- Title
Irreversible Site-Specific Hydrazinolysis of Proteins by Use of Sortase.
- Authors
Li, Yi‐Ming; Li, Yi‐Tong; Pan, Man; Kong, Xiu‐Qi; Huang, Yi‐Chao; Hong, Zhang‐Yong; Liu, Lei
- Abstract
Sortase-mediated hydrazinolysis of proteins with hydrazine or its derivatives was developed for the production of recombinant protein hydrazides. This process provides an alternative approach for protein semisynthesis through the use of recombinant protein hydrazides as thioester surrogates. It also provides an alternative method for C-terminal modification of proteins with functional units as well as for the preparation of C-to-C fusion proteins.
- Subjects
HYDRAZONES; CHEMICAL reactions; SORTASES; HYDRAZINE; PROTEIN synthesis; C-terminal binding proteins; HYDROLYSIS
- Publication
Angewandte Chemie International Edition, 2014, Vol 53, Issue 8, p2198
- ISSN
1433-7851
- Publication type
Article
- DOI
10.1002/anie.201310010