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- Title
Identification of Hypoxic Cells Using an Organotellurium Tag Compatible with Mass Cytometry.
- Authors
Edgar, Landon J.; Vellanki, Ravi N.; Halupa, Adrienne; Hedley, David; Wouters, Bradly G.; Nitz, Mark
- Abstract
Mass cytometry (MC) offers unparalleled potential for the development of highly parameterized assays for characterization of single cells within heterogeneous populations. Current reagents compatible with MC analysis employ antibody-metal-chelating polymer conjugates to report on the presence of biomarkers. Here, we expand the utility of MC by developing the first activity-based probe designed specifically for use with the technology. A compact MC-detectable telluroether is linked to a bioreductively sensitive 2-nitroimidazole scaffold, thereby generating a probe sensitive to cellular hypoxia. The probe exhibits low toxicity and is able to selectively label O2-deprived cells. A proof-of-concept experiment employing metal-bound DNA intercalators demonstrates that a heterogeneous mixture of cells with differential exposure to O2 can be effectively discriminated by the quantity of tellurium-labeling. The organotellurium reagents described herein provide a general approach to the development of a large toolkit of MC-compatible probes for activity-based profiling of single cells.
- Subjects
ORGANOTELLURIUM compounds; CYTOMETRY; LIGAND exchange chromatography; BIOMARKERS; NITROIMIDAZOLES; TELLURIUM
- Publication
Angewandte Chemie International Edition, 2014, Vol 53, Issue 43, p11473
- ISSN
1433-7851
- Publication type
Article
- DOI
10.1002/anie.201405233