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- Title
Persistence and Biodegradation of Quinalphos Using Soil Microbes.
- Authors
Kaur Dhanjal, Noorpreet Inder; Kaur, Paramjeet; Sud, Dhiraj; Cameotra, Swaranjit Singh
- Abstract
The present study reports the degradation of the persistent and toxic organophosphate, quinalphos, by employing microorganisms that were already members of the natural soil community for degradation. Bacillus and Pseudomonas spp., both of which are capable of degrading quinalphos from aqueous streams, were isolated from different contaminated soils. Batch experiments were performed to determine the natural and induced biodégradation of quinalphos in the aqueous medium. The rate of degradation was analyzed through determination of residual concentration using UV-Vis spectrophotometer and high-performance liquid chromatography. A single peak of a metabolite was observed on the 160th day, and identified as dihydroxy quinalphos oxon by mass spectrometry. The presence of quinalphos and its metabolite in water over an extended period prompted the authors to investigate its induced biodégradation using indigenous microbes extracted from soil. For biodégradation studies, the isolated microbes were inoculated into minimal media with quinalphos for 17 days. The results revealed that >80% of quinalphos was degraded in 17 days in the presence of isolated microbes, and no metabolite was observed during the biodégradation process.
- Subjects
SOIL microbiology; BIODEGRADATION; QUINALPHOS; LIQUID chromatography; PSEUDOMONADACEAE; ARABLE land
- Publication
Water Environment Research (10614303), 2014, Vol 86, Issue 5, p457
- ISSN
1061-4303
- Publication type
Article
- DOI
10.2175/106143013X13706200598514