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- Title
Characterization of the E-cadherin/catenin complex in colorectal carcinoma cell lines.
- Authors
El-Bahrawy, Mona; Poulsom, S. Richard; Rowan, Andrew J.; Tomlinson, Ian T.; Alison, Malcolm R.
- Abstract
The E-cadherin/catenin complex is a prime mediator of cell–cell adhesion. APC mutations can result in loss of β-catenin downregulation and an accumulation of β-catenin in the cell. β- CATENIN mutations can have a similar effect. The aim of this study was to investigate the effect of β- CATENIN and APC mutations on the expression and assembly of the E-cadherin/catenin complex. Five colorectal carcinoma cell lines with different APC and β- CATENIN gene status were selected and mutations were confirmed. The expression of members of the E-cadherin/catenin complex was studied by immunohistochemistry and Western blotting. Complex assembly was investigated by immunoprecipitation. It is shown that E-cadherin and catenins are expressed in colorectal carcinoma cell lines with the predominant complex assembly being E-cadherin/β-catenin/α-catenin. The subcellular distribution of the proteins is influenced by cell–cell contact, resulting in membranous localization. The expression and assembly of the E-cadherin/catenin complex does not appear to be affected by the presence of APC and or β- CATENIN mutations.
- Subjects
COLON cancer; CANCER cells; GENETIC mutation; CELLULAR pathology; CELL adhesion; CELL communication
- Publication
International Journal of Experimental Pathology, 2004, Vol 85, Issue 2, p65
- ISSN
0959-9673
- Publication type
Article
- DOI
10.1111/j.0959-9673.2004.0371.x