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- Title
TNF<sub>α</sub> Primes Polymorphonuclear Leukocytes for an Enhanced Respiratory Burst to a Similar Extent As Bacterial Lipopolysaccharide.
- Authors
Schopf, Rudolf E.; Keller, Regine; Rehder, Marianne; Benes, Peter; Kallinowski, Fritz; Vaupel, Peter
- Abstract
We examined whether preincubating polymorphonuclear leukocytes (PMN) with TNFα would result in an enhanced respiratory burst upon subsequent stimulation by various agents. Bacterial lipopolysaccharide (LPS), a known primer of PMN, was used as control. We found that both LPS (0.01 to 10.0 μg/ml) and recombinant TNFα (0.001 to 1.0μg/ml) act as direct stimulants of PMN as measured by chemiluminescence. Sixty minutes of preincubation of PMN with 1 μ/ml TNFα or 10 μg/ml LPS resulted in similar priming for the respiratory burst elicited by opsonized zymosan, phorbol myristate acetate, zymosan, zymosan-activated serum, aggregated immunoglobulin, and f-met-leu-phe (FMLP) depending on the method of measurement used, i.e., chemiluminescence, production of O2-, and H2O2. Priming with TNFα for an enhanced response to stimulation by FMLP could be abrogated by anti-TNFα antibody. Cell-surface receptor numbers and binding-affinity constants for FMLP remained stable under conditions leading to priming. We conclude that TNFα is able to prime PMN for an enhanced respiratory burst to a similar extent as with LPS. Because PMN cell-surface receptors for FMLP are unaltered by priming, the enhanced respiratory burst seems to be due to changes in intracellular metabolism.
- Subjects
NEUTROPHILS; LEUCOCYTES; ENDOTOXINS; ZYMOSAN; CELLS; CHEMILUMINESCENCE
- Publication
Journal of Investigative Dermatology, 1990, Vol 95, p216S
- ISSN
0022-202X
- Publication type
Article
- DOI
10.1111/1523-1747.ep12875802