We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Comparative genomic and phylogeographic analysis of Mycobacterium leprae.
- Authors
Monot, Marc; Honoré, Nadine; Garnier, Thierry; Zidane, Nora; Sherafi, Diana; Paniz-Mondolfi, Alberto; Matsuoka, Masanori; Taylor, G. Michael; Donoghue, Helen D.; Bouwman, Abi; Mays, Simon; Watson, Claire; Lockwood, Diana; Khamispour, Ali; Dowlati, Yahya; Shen Jianping; Rea, Thomas H.; Vera-Cabrera, Lucio; Stefani, Mariane M.; Banu, Sayera
- Abstract
Reductive evolution and massive pseudogene formation have shaped the 3.31-Mb genome of Mycobacterium leprae, an unculturable obligate pathogen that causes leprosy in humans. The complete genome sequence of M. leprae strain Br4923 from Brazil was obtained by conventional methods (6× coverage), and Illumina resequencing technology was used to obtain the sequences of strains Thai53 (38× coverage) and NHDP63 (46× coverage) from Thailand and the United States, respectively. Whole-genome comparisons with the previously sequenced TN strain from India revealed that the four strains share 99.995% sequence identity and differ only in 215 polymorphic sites, mainly SNPs, and by 5 pseudogenes. Sixteen interrelated SNP subtypes were defined by genotyping both extant and extinct strains of M. leprae from around the world. The 16 SNP subtypes showed a strong geographical association that reflects the migration patterns of early humans and trade routes, with the Silk Road linking Europe to China having contributed to the spread of leprosy.
- Subjects
MYCOBACTERIUM; MYCOBACTERIAL diseases; PATHOGENIC bacteria; HANSEN'S disease; HUMAN genome; NUCLEOTIDE sequence
- Publication
Nature Genetics, 2009, Vol 41, Issue 12, p1282
- ISSN
1061-4036
- Publication type
Article
- DOI
10.1038/ng.477