We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Dendritic Cell Vaccine Therapy by Immunization with Fusion Cells of Interleukin-2 Gene-Transduced, Spleen-Derived Dendritic Cells and Tumour Cells.
- Authors
Ogawa, F.; Iinuma, H.; Okinaga, K.
- Abstract
We examined the preventive and therapeutic effects of fusion cells prepared from spleen-derived dendritic cells (DCs) transduced with the interleukin-2 (IL-2) gene and QRsP fibrosarcoma cells in a mouse lung metastasis model. The IL-2 or LacZ gene was introduced into spleen-derived DCs using an adenoviral vector. Irradiated QRsP tumour cells were fused with IL-2 gene-transduced DCs (fusion/IL-2) or LacZ gene-transduced DCs (fusion/LacZ) by polyethylene glycol. These fusion cells expressed major histocompatibility complexes (MHC) class I and II, CD86, CD11c and CD80α. Splenocytes from mice vaccinated with fusion cells showed increased production of interferon-γ (IFN-γ) and cytotoxic T-lymphocyte (CTL) activity as compared with those vaccinated with DCs or tumour cells alone, and CTL levels were higher in fusion/IL-2-vaccinated mice than in fusion/LacZ-vaccinated mice. In our experiments on the protective and therapeutic effects on lung metastasis, mice vaccinated with fusion/IL-2 fusion/ LacZ or fusion showed a significant reduction in pulmonary metastasis compared with those given DCs, tumour or phosphate-buffered saline. The introduction of the IL-2 gene into fusion cells produced more potent preventive and therapeutic effects. These results suggest that immunization with fusion cells prepared from spleen-derived DCs and tumour cells is capable of inducing preventive and therapeutic anti-tumour immunity against lung metastasis, and modification by the IL-2 gene may increase anti-tumour efficacy.
- Subjects
DENDRITIC cells; ANTIGEN presenting cells; LYMPHOID tissue; VACCINATION; INTERLEUKINS; IMMUNOLOGY
- Publication
Scandinavian Journal of Immunology, 2004, Vol 59, Issue 5, p432
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.0300-9475.2004.01411.x