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- Title
P64k Meningococcal Protein as Immunological Carrier for Weak Immunogens.
- Authors
González; Alvarez; Caballero; Viña; Guillén; Silva
- Abstract
Previously, the P64k meningococcal protein, an antigen of 64 kDa expressed in Escherichia coli, has been extensively characterized. We have successfully conjugated several synthetic peptides and meningococcal group C polysaccharide to P64k. In three out of four model peptides, the murine humoral immune response against the homologous peptide, evaluated after three doses of conjugate, was higher in the animals immunized with the coupled peptide than in those that received free peptide. The fourth and largest was immunogenic by itself. Similarly, the antigroup C polysaccharide levels reached by conjugated polysaccharide were significantly higher than those produced against unconjugated polysaccharide. As a carrier for one of the peptides, P64k was compared with bovine serum albumin (BSA) and tetanus toxoid (TT), being able to induce slightly higher or similar antipeptide antibody levels than these well-establish protein carriers. Our results suggest that recombinant P64k protein could be a readily available immunological carrier, as efficient as other commonly used large carrier molecules.
- Subjects
CARRIER proteins; IMMUNOGENETICS
- Publication
Scandinavian Journal of Immunology, 2000, Vol 52, Issue 2
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1046/j.1365-3083.2000.00788.x