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- Title
Ig-Isotype Patterns of Primary and Secondary B Cell Responses to Plasmodium Chabaudi Chabaudi Correlate with IFN-γ and IL-4 Cytokine Production and with CD45RB Expression by CD4<sup>+</sup> Spleen Cells.
- Authors
D'Império Lima, M. R.; Alvarez, J. M.; Furtado, G. C.; Kipnis, T. L.; Coutinho, A.; Minóprio, P.
- Abstract
In this work, the authors analysed T and B lymphocyte subsets and cytokine production in the spleen of BALB/c mice during polyclonal lymphocyte activation (primary infection) and parasite-specific response to <em>Plasmodium chabaudi chabaudi</em> (secondary infection). The secondary response was evaluated in fully immunoprotected animals, 60 days after a chloroquine-cured infection. The authors observed that in polyclonal lymphocyte activation antibody-secreting cells of all isotypes increased, with predominance of IgG2a and IgG3 classes. At that time, IFN-γ was largely produced, but IL-4/IL-5 were just slightly enhanced. In mice re-infected after 60 days, the Ig-isotype pattern was restricted to IgG1 and only IL-4/IL-5 were produced. In both responses, however, the levels of IL-2 were greatly reduced, while those of IL-10 were enhanced to similar levels. The different involvement of Th1 and Th2 cells in both responses was confirmed through analysis of CD45RB expression by CD4+ cells. The authors observed that CD45RBhigh cells were the major CD4+ subpopulation in primary infected mice, while CD45RBlow cells predominated in 60 days re-infected animals. Moreover, the great majority of activated (large) CD4+ cells in the primary infection belonged to the CD45RBhigh subset, while alter re-infection most of the CD4+ large had a CD45RBlow phenotype.
- Subjects
CELLS; MICE; LYMPHOCYTE transformation; B cells; CELLULAR immunity; HEMATOPOIETIC system; LYMPHOID tissue; PLASMODIIDAE; T cells; TH2 cells; GENOTYPE-environment interaction
- Publication
Scandinavian Journal of Immunology, 1996, Vol 43, Issue 3, p263
- ISSN
0300-9475
- Publication type
Article