We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
T-Cell Receptor β-Chain Gene Rearrangements of T-Cell Populations Expanded from Multiple Sites of Synovial Tissue obtained from a Patient with Rheumatoid Arthritis.
- Authors
van Laar, J. M.; Miltenburg, A. M. M.; Verdonk, M. J. A.; Daha, M. R.; de Vries, R. R. P.; van den Elsen, P. J.; Breedveld, E. C.
- Abstract
In this study T-cell receptor (TcR) β-chain gene rearrangements of T-cell lines prepared from multiple sites (n = 92) of synovial tissue derived from both knees of a patient with rheumatoid arthritis were analysed. In the majority of T-cell lines, dominant TcR β-chain gene rearrangements were detected, involving Cβ1 as well as Cβ2. The dominant rearrangement patterns of T-cell lines from different tissue fragments showed significant variability, but some of the DNA restriction fragments were shared by T-cell lines from multiple sites in both knees. The latter observation suggests that identical T-cell clones may be present at different sites in the synovial tissue and in different joints. However, since many T-cell lines yielded different rearrangement patterns, these data also indicate considerable heterogeneity of T-cells in the joints. Apart from theoretical implications, this TcR heterogeneity of T-cells within an individual patient also has practical consequences for studies on synovial T cells obtained by biopsy.
- Subjects
ARTHRITIS; CELL culture; CELL membranes; AUTOIMMUNE diseases; JOINT diseases; CELL proliferation
- Publication
Scandinavian Journal of Immunology, 1992, Vol 35, Issue 2, p187
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1992.tb02849.x