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- Title
Interferon β2/b-Cell Stimulating Factor 2/ Interleukin 6 Affects Glycosylation of Acute Phase Proteins in Human Hepatoma Cell Lines.
- Authors
Mackiewicz, A.; Kushner, I.
- Abstract
Undefined monocyte-derived cytokines have previously been shown to affect glycan processing glycoproteins secreted by human hepatoma cell lines. Hep 3B cells, when incubated with the cytokine interferon β2/B-cell stimulating factor 2/interleukin 6, secreted forms of α1-protease inhibitor, ceruplasmin, and α-feroprotein with increased reactivity with concanavalin A (Con A) while incubation of HEp G2 cells with this cytokine led to secretion of forms of these proteins with decreased reactivity with Con A, reflecting changes in their oligosaccharide chains. The difference in response of these two transformed cell lines to this cytokine undoubtedly reflects differences in this intracellular glycan processing mechanisms. Changes in glycosylation patterns were dissociated from changes in rate of synthesis: this cytokine caused increased synthesis of α1-protease inhibitor and ceruloplasmin, and decreased synthesis of α-fetoprotein in both cell lines.
- Subjects
PROTEINS; CELL culture; CELL lines; CELLULAR immunity; ANTIVIRAL agents; GLYCOPROTEINS
- Publication
Scandinavian Journal of Immunology, 1989, Vol 29, Issue 3, p265
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1989.tb01124.x