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- Title
Spontaneous Reverse Haemolytic Plaque Formation II. The Role of T Cells and Monocytes in Regulating Immunoglobulin Secretion by Human Peripheral Blood B Cells.
- Authors
Librach, C. L.; Burns, G. F.
- Abstract
A sensitive reverse haemolytic plaque assay was used on freshly isolated blood cells from normal human subjects to show that T lymphocytes and monocytes were both necessary for immunoglobulin production by unstimulated B cells cultured only for the time necessary to form plaques. When lymphocyte preparations were fully depleted of T cells by E-rosette formation overnight on ice followed by Ficoll-Hypaque centrifugation, the number of plaque-forming cells was reduced by up to 94% this reduction was reversed by the replacement of T cells, although excess T cells suppressed plaque formation. Moreover, when T-cell function was blocked by 10–1000 ng of two monoclonal anti-T-cell antibodies, OKT3 or UCHT1. this significantly reduced or abolished spontaneous IgG plaque, and higher concentrations of either OKT3 or UCHT1 reduced the numbers of IgA and IgM plaques formed by B cells. The role of monocytes in spontaneous plaque formation was investigated. The removal of plastic-adherent cells from mononuclear cell preparations did not consistently result in a reduction in the numbers of plaques, but complement-mediated lysis of monocytes with either of two monoclonal antibodies with specificity for rnonocytes, OKM1 and FMC1T, reduced by 50% the number of IgG, IgA and IgM plaques. This effect was reversed by addition of as few as 1% plastic-adherent cells. Decreased plaque formation by B cells, resulting from either blocking of T-cell function with monoclonal antibody or complement-mediated lysis of monocytes, or both, was fully reversed by soluble factors present in cell-free conditioned medium from lectin-activated T cells. Thus spontaneous plaque formation by human peripheral blood B cells requires T cells and a small number of monocytes, and the major function of these cells is to help B cells by the production of soluble factors.
- Subjects
BLOOD cells; HEMOLYTIC plaque technique; HEMOLYSIS &; hemolysins; PLAQUE assay technique; LYMPHOCYTES; MONOCYTES; IMMUNOGLOBULINS; B cells; ANTIGEN presenting cells
- Publication
Scandinavian Journal of Immunology, 1983, Vol 17, Issue 2, p171
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1983.tb00780.x