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- Title
Oral immunization of mice with Saccharomyces cerevisiae expressing a neutralizing epitope of Apx IIA exotoxin from Actinobacillus pleuropneumoniae induces systemic and mucosal immune responses.
- Authors
Shin, Min‐Kyoung; Lee, Won‐Jung; Jung, Myung Hwan; Cha, Seung‐Bin; Shin, Seung‐Won; Yoo, Anna; Kim, Dae‐Hyuk; Yoo, Han Sang
- Abstract
ABSTRACT An oral delivery system based on ApxIIA#5-expressed on Saccharomyces cerevisiae was studied for its potential to induce immune responses in mice. Murine bone marrow-derived dendritic cells (DCs) stimulated in vitro with ApxIIA#5-expressed on S. cerevisiae upregulated the expression of maturation and activation markers, leading to production of tumor necrosis factor-α, interleukin (IL)-1β, IL-12p70 and IL-10. Presentation of these activated DCs to cluster of differentiation CD4+ T cells collected from mice that had been orally immunized with the ApxIIA#5-expressed on S. cerevisiae elicited specific T-cell proliferation. In addition, the orally immunized mice had stronger antigen-specific serum IgG and IgA antibody responses and larger numbers of antigen-specific IgG and IgA antibody-secreting cells in their spleens, Peyer's patches and lamina propria than did those immunized with vector-only S. cerevisiae or those not immunized. Furthermore, oral immunization induced T helper 1-type immune responses mediated via increased serum concentrations of IgG2a and an increase predominantly of IFN-γ-producing cells in their spleens and lamina propria. Our findings suggest that surface-displayed ApxIIA#5-expressed on S. cerevisiae may be a promising candidate for an oral vaccine delivery system for eliciting systemic and mucosal immunity.
- Subjects
IMMUNIZATION; SACCHAROMYCES cerevisiae; FUNGAL gene expression; EXOTOXIN; ACTINOBACILLUS pleuropneumoniae; IMMUNE response; LABORATORY mice
- Publication
Microbiology & Immunology, 2013, Vol 57, Issue 6, p417
- ISSN
0385-5600
- Publication type
Article
- DOI
10.1111/1348-0421.12053