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- Title
Automated microfluidic devices integrating solid-phase extraction, fluorescent labeling, and microchip electrophoresis for preterm birth biomarker analysis.
- Authors
Sahore, Vishal; Sonker, Mukul; Nielsen, Anna V.; Knob, Radim; Kumar, Suresh; Woolley, Adam T.
- Abstract
We have developed multichannel integrated microfluidic devices for automated preconcentration, labeling, purification, and separation of preterm birth (PTB) biomarkers. We fabricated multilayer poly(dimethylsiloxane)-cyclic olefin copolymer (PDMS-COC) devices that perform solid-phase extraction (SPE) and microchip electrophoresis (μCE) for automated PTB biomarker analysis. The PDMS control layer had a peristaltic pump and pneumatic valves for flow control, while the PDMS fluidic layer had five input reservoirs connected to microchannels and a μCE system. The COC layers had a reversed-phase octyl methacrylate porous polymer monolith for SPE and fluorescent labeling of PTB biomarkers. We determined μCE conditions for two PTB biomarkers, ferritin (Fer) and corticotropin-releasing factor (CRF). We used these integrated microfluidic devices to preconcentrate and purify off-chip-labeled Fer and CRF in an automated fashion. Finally, we performed a fully automated on-chip analysis of unlabeled PTB biomarkers, involving SPE, labeling, and μCE separation with 1 h total analysis time. These integrated systems have strong potential to be combined with upstream immunoaffinity extraction, offering a compact sample-to-answer biomarker analysis platform.
- Subjects
PREMATURE labor; BIOLOGICAL tags; MICROFLUIDIC devices; SOLID phase extraction; MICROCHIP electrophoresis
- Publication
Analytical & Bioanalytical Chemistry, 2018, Vol 410, Issue 3, p933
- ISSN
1618-2642
- Publication type
Article
- DOI
10.1007/s00216-017-0548-7