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- Title
Reproducibility of retention time and peak area in comprehensive two-dimensional liquid chromatography.
- Authors
Elsner, Victoria; Wulf, Volker; Wirtz, Michaela; Schmitz, Oliver
- Abstract
Comprehensive two-dimensional liquid chromatography is used to separate anionic, nonionic, and amphoteric surfactants by substance class, alkyl chain distribution, and degree of ethoxylation. A nearly orthogonal system with a hydrophilic interaction chromatography (HILIC) phase in the first and a reversed-phase material in the second dimension is applied to generate a separation with maximum peak capacity. The potential of the developed method is demonstrated by the reproducibility of retention time and peak area, which shows standard deviations less than 5 % and the analysis of real samples. An external calibration and the standard addition method were applied to determine unknown concentrations for the alkyl chain homologues of a betaine and for one ethoxylate (EO) homologue of a fatty alcohol ethoxylate in a sample mixture.
- Subjects
LIQUID chromatography; SURFACE active agents; ETHOXYLATION; HYDROPHILIC interaction liquid chromatography; STANDARD deviations; CALIBRATION
- Publication
Analytical & Bioanalytical Chemistry, 2015, Vol 407, Issue 1, p279
- ISSN
1618-2642
- Publication type
Article
- DOI
10.1007/s00216-014-8090-3