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- Title
Effect of ancymidol on cell wall metabolism in growing maize cells.
- Authors
Hernández-Altamirano, J. Mabel; Largo-Gosens, Asier; Martínez-Rubio, Romina; Pereda, Diego; Álvarez, Jesús M.; Acebes, José L.; Encina, Antonio; García-Angulo, Penélope
- Abstract
Main conclusion: Ancymidol inhibits the incorporation of cellulose into cell walls of maize cell cultures in a gibberellin-independent manner, impairing cell growth; the reduction in the cellulose content is compensated with xylans.Ancymidol is a plant growth retardant which impairs gibberellin biosynthesis. It has been reported to inhibit cellulose synthesis by tobacco cells, based on its cell-malforming effects. To ascertain the putative role of ancymidol as a cellulose biosynthesis inhibitor, we conducted a biochemical study of its effect on cell growth and cell wall metabolism in maize cultured cells. Ancymidol concentrations ≤ 500 µM progressively reduced cell growth and induced globular cell shape without affecting cell viability. However, cell growth and viability were strongly reduced by ancymidol concentrations ≥ 1.5 mM. The <italic>I</italic>50 value for the effect of ancymidol on FW gain was 658 µM. A reversal of the inhibitory effects on cell growth was observed when 500 µM ancymidol-treated cultures were supplemented with 100 µM GA3. Ancymidol impaired the accumulation of cellulose in cell walls, as monitored by FTIR spectroscopy. Cells treated with 500 µM ancymidol showed a ~ 60% reduction in cellulose content, with no further change as the ancymidol concentration increased. Cellulose content was partially restored by 100 µM GA3. Radiolabeling experiments confirmed that ancymidol reduced the incorporation of [14C]glucose into α-cellulose and this reduction was not reverted by the simultaneous application of GA3. RT-PCR analysis indicated that the cellulose biosynthesis inhibition caused by ancymidol is not related to a downregulation of <italic>ZmCesA</italic> gene expression. Additionally, ancymidol treatment increased the incorporation of [3H]arabinose into a hemicellulose-enriched fraction, and up-regulated <italic>ZmIRX9</italic> and <italic>ZmIRX10L</italic> gene expression, indicating an enhancement in the biosynthesis of arabinoxylans as a compensatory response to cellulose reduction.
- Subjects
PLANT cell walls; BIOSYNTHESIS; ANCYMIDOL; UNICONAZOLE; GENE expression
- Publication
Planta: An International Journal of Plant Biology, 2018, Vol 247, Issue 4, p987
- ISSN
0032-0935
- Publication type
Article
- DOI
10.1007/s00425-018-2840-y