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- Title
The effect of preincubation during egg storage on embryo development of young parent stock.
- Authors
Tesarova, M.; Lason, M.; Hampel, D.; Foltyn, M.; Lichovnikova, M.
- Abstract
Freshly laid eggs from parent stock of Ross 308 at the age 31 weeks were used in the experiment. They were divided into five groups; fresh eggs (WP - without preincubation), preincubated once per 4 h (PS1 - one short preincubation), preincubated twice for 4 h (PS2 - two short preincubations), preincubated once per 8 h (PL1 - one long preincubation) and preincubated twice per 8 h (PL2 - tow long preincubation). Preincubated eggs were incubated after 5 and 10 days of storage for 4 or 8 h. In fresh eggs and after each treatment 60 eggs were examined for embryo development. After distinguish the egg status (fertilized or not), blastoderm was isolated and cleaned off the eggwhite and yolk, and then analyzed under the stereo microscope and staged according to Eyal-Giladi and Kochav (EGK, Roman numerals) procedure. However not all embryos were successfully isolated. The following numbers of embryos were successfully determined: WP - 23, PS1 - 25, PS2 - 10, PL1 - 20, PL2 - 8. Because of discrete nature of data with low number of variants, nonparametric Kruskal-Wallis ANOVA with subsequent pairwise comparisons was used for evaluation. Two statistically different groups were detected; the first one was created by WP (median X) and PS1 (median XI), the second one by PL1 (median XII - XIII), PS2 (median XIII) and PL2 (median XIII). It seems that second long term (8 h) preincubation does not significantly improve embryo development. Anyway this should be confirmed by staging higher number of embryos.
- Subjects
EGG storage; EGG incubation; BLASTODERM
- Publication
European Poultry Science / Archiv für Geflügelkunde, 2017, Vol 81, Issue 204, p36
- ISSN
0003-9098
- Publication type
Abstract
- DOI
10.1399/eps.2017.204