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- Title
Thermosensitive Hydrogel PEG–PLGA–PEG Enhances Engraftment of Muscle-derived Stem Cells and Promotes Healing in Diabetic Wound.
- Authors
Pui Yan Lee; Cobain, Erin; Huard, Johnny; Huang, Leaf
- Abstract
Regenerating new tissue using cell transplantation has relied on successful cell engraftment in the host; however, cell engraftment into the diabetic skin wound is not as successful as in many other tissues. We used a biodegradable and biocompatible triblock co-polymer poly(ethylene glycol-b-[dl-lactic acid-co-glycolic acid]-b-ethylene glycol) (PEG–PLGA–PEG), which forms a thermosensitive hydrogel, as a wound dressing and scaffold. We found that the thermosensitive hydrogel increased the engraftment of muscle-derived stem cells (MDSCs) by 20- to 30-fold until day 20, when the wound was completely closed in a db/db genetically diabetic mouse model. At day 9, 30% of the transplanted MDSCs were found to remain, and 15% remained at day 20 after transplantation. The increased engraftment resulted in enhanced wound healing, as indicated by the wound closure rate, epithelium migration, and collagen deposition. Using MDSCs stably expressing β-gal and immunofluorescence, we found that 25% of MDSCs differentiated into fibroblasts, 10% into myofibroblasts, and 10% into endothelial cells. We conclude that using the thermosensitive hydrogel as a scaffold increased the engraftment of MDSCs, which leads to improved diabetic wound healing, possibly by retaining the cells at the wound site for longer.Molecular Therapy (2007) 15 6, 1189–1194. doi:10.1038/sj.mt.6300156
- Subjects
HYDROGELS; CELL transplantation; STEM cells; PEOPLE with diabetes; DIABETES; MEDICAL research
- Publication
Molecular Therapy, 2007, Vol 15, Issue 6, p1189
- ISSN
1525-0016
- Publication type
Article
- DOI
10.1038/sj.mt.6300156