We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Krebs Cycle Intermediates Protective against Oxidative Stress by Modulating the Level of Reactive Oxygen Species in Neuronal HT22 Cells.
- Authors
Kenta Sawa; Takumi Uematsu; Yusuke Korenaga; Ryuya Hirasawa; Masatoshi Kikuchi; Kyohei Murata; Jian Zhang; Xiaoqing Gai; Sakamoto, Kazuichi; Tomoyuki Koyama; Takumi Satoh
- Abstract
Krebs cycle intermediates (KCIs) are reported to function as energy substrates in mitochondria and to exert antioxidants effects on the brain. The present study was designed to identify which KCIs are effective neuroprotective compounds against oxidative stress in neuronal cells. Here we found that pyruvate, oxaloacetate, and α-ketoglutarate, but not lactate, citrate, iso-citrate, succinate, fumarate, or malate, protected HT22 cells against hydrogen peroxide-mediated toxicity. These three intermediates reduced the production of hydrogen peroxide-activated reactive oxygen species, measured in terms of 2',7'-dichlorofluorescein diacetate fluorescence. In contrast, none of the KCIs-used at 1 mM-protected against cell death induced by high concentrations of glutamate-another type of oxidative stress-induced neuronal cell death. Because these protective KCIs did not have any toxic effects (at least up to 10 mM), they have potential use for therapeutic intervention against chronic neurodegenerative diseases.
- Subjects
KREBS cycle; OXIDATIVE stress; ANTIOXIDANTS; ALPHA-ketoglutarate dioxygenase; HYDROGEN peroxide; CELL death; PREVENTION; THERAPEUTICS
- Publication
Antioxidants, 2017, Vol 6, Issue 1, p21
- ISSN
2076-3921
- Publication type
Article
- DOI
10.3390/antiox6010021