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- Title
Kinetic and inhibitory study of partially purified lipoxygenase from epilepsy patient’s serum.
- Authors
Saleh Al-Fayyadh, Ahmed Ali; Abo Shahine, Nashwan Ibrahem
- Abstract
This research was included partially purification of lipoxygenase (LOX) from serum of patients with epilepsy using ammonium sulfate precipitation, dialysis and ion-exchange chromatography with specific activity (0.751 U / mg), (0.935 U/mg) and (2.60 U/mg) respectively. Optimum conditions for enzyme activity were determined. The best enzyme activity was showed at 7 minutes of incubation time, 3 minutes of reaction time, pH = 7, temperature 40Cº and finally substrate concentration (linoleic acid) was (1.2 mmol/L). By applying Michaelis-Menten and Lineweaver-Burk Equations, Michaelis-Menten constant (km) and Vmax values were found 0.3 (mM) and (0.9 U/ml) respectively. Also this study included the effect of some antiepileptic drugs such as valproic acid, carbamazepine and acetozolamide. Acetazolamide showed the highest inhibition of lipooxygenase activity)٪96.2(, valproic acid(95.6) and Carbamazapine)٪95.1( . Inhibition type was studied and the result showed noncompetitive inhibition by using Lineweaver-Burke plot for all above drugs.
- Subjects
LIPOXYGENASES; BLOOD serum analysis; AMMONIUM sulfate; MICHAELIS-Menten equation; ANTICONVULSANTS; PEOPLE with epilepsy
- Publication
Journal of Education & Science, 2021, Vol 30, Issue 1, p58
- ISSN
1812-125X
- Publication type
Article
- DOI
10.33899/edusj.2020.127778.1093