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- Title
利用逆转录环介导恒温扩增技术建立新型冠状病毒核酸快速检测方法.
- Authors
李 欢; 徐秋月; 王云娟; 苏 洋; 唐睿珠
- Abstract
Objective To establish a rapid detection method for the nucleic acid of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) based on the reverse transcription loop mediated isothermal amplification (RT-LAMP). Method The RT-LAMP primers were designed according to the conservative region of N and ORF1ab gene of SARS-CoV-2. And the specificity,the minimum detection limit,the anti-interference ability and the best reaction time of the system were evaluated. Result Artificial synthesized SARS-CoV-2 nucleic acid samples were tested,and the results were consistent with the expected results.It confirmed the feasibility of the detection system. Conclusion The reaction system using RT-LAMP for the rapid detection of SARS-CoV-2 nucleic acid has the high specificity and sensitivity,and is suitable for the border areas with limited conditions.
- Publication
Journal of Kunming Medical University / Kunming Yike Daxue Xuebao, 2021, Vol 42, Issue 9, p25
- ISSN
2095-610X
- Publication type
Article
- DOI
10.12259/j.issn.2095-610X.S20210904