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- Title
Investigation of the Relationship between the Lectin Activity of Azospirilla and Their α-Glucosidase, β-Glucosidase, and β-Galactosidase.
- Authors
Alen'kina, S. A.; Nikitina, V. E.; Borisova-Golovko, M. V.
- Abstract
The activities of α-glucosidase, β-glucosidase, and β-galactosidase were studied during the isolation and purification of lectins from Azospirillum brasilenseSp7 and Azospirillum lipoferum59b cells. These enzymatic activities were revealed in crude extracts of surface proteins, protein fraction precipitated with ammonium sulfate or ethanol–acetone mixture, and protein fraction obtained by gel filtration on Sephadex G-75. The distribution of the enzymes between different protein fractions varied for the azospirilla studied. The cofunction of the A. brasilenseSp7 lectin and β-galactosidase on the cell surface is assumed. A strong interaction between the A. lipoferum59b lectin and glucosidases was revealed. The lectin from A. lipoferum59b may possess saccharolytic activity.
- Subjects
AZOSPIRILLUM; LECTINS; GLUCOSIDASES; RHIZOBACTERIA; BACTERIOLOGY; MICROBIOLOGY
- Publication
Microbiology (00262617), 2001, Vol 70, Issue 5, p557
- ISSN
0026-2617
- Publication type
Article
- DOI
10.1023/A:1012308205568