We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Cell wall glycanases and their activity against the hemicelluloses from pine hypocotyls.
- Authors
Acebes, José Luis; Zarra, Ignacio
- Abstract
Glycanases associated with the cell walls of Pinus pinaster Aiton from Northern Spain (29QT638, altitude 1000 m) were extracted with a 1 M LiCl solution, and their effect on the hemicellulosic polysaccharides from the same material was studied. The extracted proteins were able to increase the amount of reducing sugars and to decrease the viscosity of the hemicellulosic polysaccharides extracted with both 4 and 24% (w/v) KOH, suggesting the presence of exo- and endo-type glycanases. The extracted proteins released arabinose, galactose and xylose from both hemicellulosic polysaccharides, and caused an important depolymerization of xyloglucan. The results indicate that the changes in the hemicellulosic polysaccharides, i.e., xyloglucan depolymerization, found during cell elongation are caused by an enzymic system associated with the cell wall.
- Subjects
SPAIN; ENZYME kinetics; CLUSTER pine; PLANT cell walls; HEMICELLULOSE; PROTEINS; POLYSACCHARIDES
- Publication
Physiologia Plantarum, 1992, Vol 86, Issue 3, p433
- ISSN
0031-9317
- Publication type
Article
- DOI
10.1111/j.1399-3054.1992.tb01340.x