We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Circle‐Seq reveals genomic and disease‐specific hallmarks in urinary cell‐free extrachromosomal circular DNAs.
- Authors
Lv, Wei; Pan, Xiaoguang; Han, Peng; Wang, Ziyu; Feng, Weijia; Xing, Xue; Wang, Qingqing; Qu, Kunli; Zeng, Yuchen; Zhang, Cailin; Xu, Zhe; Li, Yi; Zheng, Tianyu; Lin, Ling; Liu, Chengxun; Liu, Xuemei; Li, Hanbo; Henriksen, Rasmus Amund; Bolund, Lars; Lin, Lin
- Abstract
Background: Extrachromosomal circular deoxyribonucleic acid (eccDNA) is evolving as a valuable biomarker, while little is known about its presence in urine. Methods: Here, we report the discovery and analysis of urinary cell‐free eccDNAs (ucf‐eccDNAs) in healthy controls and patients with advanced chronic kidney disease (CKD) by Circle‐Seq. Results: Millions of unique ucf‐eccDNAs were identified and comprehensively characterised. The ucf‐eccDNAs are GC‐rich. Most ucf‐eccDNAs are less than 1000 bp and are enriched in four pronounced peaks at 207, 358, 553 and 732 bp. Analysis of the genomic distribution of ucf‐eccDNAs shows that eccDNAs are found on all chromosomes but enriched on chromosomes 17, 19 and 20 with a high density of protein‐coding genes, CpG islands, short interspersed transposable elements (SINEs) and simple repeat elements. Analysis of eccDNA junction sequences further suggests that microhomology and palindromic repeats might be involved in eccDNA formation. The ucf‐eccDNAs in CKD patients are significantly higher than those in healthy controls. Moreover, eccDNA with miRNA genes is highly enriched in CKD ucf‐eccDNA. Conclusions: This work discovers and provides the first deep characterisation of ucf‐eccDNAs and suggests ucf‐eccDNA as a valuable noninnvasive biomarker for urogenital disorder diagnosis and monitoring.
- Subjects
EXTRACHROMOSOMAL DNA; CIRCULAR DNA; DNA; CIRCULAR RNA; CHRONIC kidney failure; CELL-free DNA; CIRCULATING tumor DNA
- Publication
Clinical & Translational Medicine, 2022, Vol 12, Issue 4, p1
- ISSN
2001-1326
- Publication type
Article
- DOI
10.1002/ctm2.817