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- Title
Applications of multiplexed immunohistochemistry/immunofluorescence and multispectral imaging technology in the field of tumor immunotherapy.
- Authors
Wenzhe Li; Xia Yuan; Bo Xu; Shuxiang Song
- Abstract
Multiplexed immunohistochemistry/fluorescence (mIHC/IF) in combination with multispectral unmixing is a novel multitarget histopathological staining and imaging technique. By simultaneously revealing expression level and spatial information for up to eight biomarkers in situ, in addition to a nuclear stain within a single formalin-fixed paraffin-embedded (FFPE) tissue section, this technology can analyze the phenotype, abundance, morphology and intercellular relationship of cells while providing statistically significant results. In recent years, technical improvements have brought new insight into mIHC/IF and multispectral imaging approaches, which have been successfully applied in the field of cancer immunotherapy, specifically in regard to tumor microenvironment research, immunotherapy drug discovery, and prognostic and metastatic risk evaluation. This review highlights the principle, workflow, advantages and disadvantages of the technology, and discusses the latest applications of mIHC/IF-based imaging technology in the field of TME-related research and immunotherapy drug discovery.
- Subjects
MULTISPECTRAL imaging; IMMUNOHISTOCHEMISTRY; IMMUNOFLUORESCENCE; IMMUNOTHERAPY; TUMOR microenvironment; BIOMARKERS
- Publication
Journal of Chinese Pharmaceutical Sciences, 2020, Vol 29, Issue 10, p734
- ISSN
1003-1057
- Publication type
Article
- DOI
10.5246/jcps.2020.10.069