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- Title
Microscale thermophoresis in the investigation of biomolecular interactions.
- Authors
Qian Wang; Jing Wang; Shuxiang Song; Guiwang Zhu; Ze Cao; Zhenming Liu
- Abstract
Accurate characterization of the interactions between biomolecules not only provides fundamental insights into cellular processes but also paves the way for drug discovery and development. With recent increases in throughput and sensitivity, biophysical technologies have become prominent tools for studying biomolecular interactions. Biophysical techniques that can reduce costs, shorten detection time, simplify the complexity of the system under analysis, and simultaneously provide high-quality data content are particularly favored. Here, we summarize the qualitative and quantitative analysis of biomolecular interactions using MicroScale Thermophoresis (MST), as well as extend the application of MST functions to explore thermodynamics, enzyme kinetics and protein folding-unfolding processes. MST has emerged as a simple and powerful biophysical approach for identifying and quantifying binding events based on the movement of molecules along microscopic temperature gradients. The advantages of MST over other competitive biophysical techniques include freedom from immobilization, rapid analysis times, lower sample consumption, and the ability to analyze binding affinities in cell lysates. This article discusses the instrumental setups, principles, experimental workflows, and examples of MST application in practice.
- Subjects
THERMOPHORESIS; ENZYME kinetics; THERMODYNAMICS; DRUG development; SYSTEM analysis; FREE energy (Thermodynamics)
- Publication
Journal of Chinese Pharmaceutical Sciences, 2020, Vol 29, Issue 9, p656
- ISSN
1003-1057
- Publication type
Article
- DOI
10.5246/jcps.2020.09.061