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- Title
Mutational probing of protein aggregates to design aggregation‐resistant proteins.
- Authors
Kamal, Mohamad Zahid; Kumar, Virender; Satyamurthi, Kundarapu; Das, Kushal Kumar; Rao, Nalam Madhusudhana
- Abstract
Characterization of amorphous protein aggregates may offer insights into the process of aggregation. Eleven single amino acid mutants of lipase (LipA of Bacillus subtilis) were subjected to temperature‐induced aggregation, and the resultant aggregates were characterized for recovery of activity in the presence of guanidinium chloride (GdmCl). Based on activity recovery profiles of the aggregates, the mutants could be broadly assigned into four groups. By including at least one mutation from each group, a mutant was generated that showed an increase of ~ 10 °C in melting temperature (Tm) compared to the wild‐type and did not aggregate even at 75 °C. This method explores characterization of amorphous protein aggregates in the presence of GdmCl and helps in identifying mutations involved in protein aggregation.
- Subjects
PROTEIN engineering; GUANIDINIUM chlorides; BACILLUS subtilis; AMINO acids; PROTEIN stability; LIPASES
- Publication
FEBS Open Bio, 2016, Vol 6, Issue 2, p126
- ISSN
2211-5463
- Publication type
Article
- DOI
10.1002/2211-5463.12003