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- Title
The therapeutic effect of Punica granatum peel extract on ionizing radiation-induced enteritis in rats.
- Authors
Toklu, Hale Z.; Sehirli, Özer; Dumlu, Melek U.; Özyurt, Hazan; Mayadagli, Alpaslan; Gökmen, Vural; Yuksel, Meral; Çetinel, Şule; Ekşioğlu-Demiralp, Emel; Yeğen, Berrak Ç.; Sener, Goksel
- Abstract
Radiation-induced enteritis is a well-recognized sequel of therapeutic irradiation. The objective of this study was to examine the potential radioprotective properties of Punica granatum peel extract (PGE) on the oxidative damage in the ileum of irradiation-induced enteritis. Sprague-Dawley rats were exposed to irradiation performed with a LIN AC producing 6 MV photons at a focus 100 cm distant from the skin. Under ketamine anaesthesia (100 mg/kg intraperitoneally), each rat received a single whole-body X-ray irradiation of 800 cGy. Animals were returned to their home cages following irradiation. Irradiated rats were pretreated orally with saline or PGE (50 mg/kg/day) for 10 days before irradiation and the daily treatments were continued for additional 10 days. After decapitation, trunk blood was collected to measure lactate dehydrogenase (LDH) and tumor necrosis factor-alpha (TNF-alpha) levels. Furthermore, oxidative burst of neutrophils and leukocyte apoptosis were assayed by flow cytometry. In the ileal tissues malondialdehyde (MDA) and glutathione (GSH) levels, myeloperoxidase (MPO) activity were evaluated. Plasma TNF-alpha, LDH levels and leukocyte apoptosis were increased in the irradiated rats as compared with the control group. Irradiation caused a significant decrease in ileal GSH level, which was accompanied by significant increases in MDA levels and MPO activity of the ileal tissue. On the other hand, PGE treatment reversed all the biochemical and histopathological alterations induced by irradiation. Our results demonstrate that PGE, via its putative antioxidant action, reduces oxidative damage in the ileal tissues of irradiated rats. Thus, the present results suggest that supplementing cancer patients with PGE may support radiotherapy by improving radiation-induced enteritis.
- Publication
Cell Membranes & Free Radical Research, 2008, Vol 1, Issue 1, p31
- ISSN
1308-416X
- Publication type
Article