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- Title
Octanoylated Ghrelin Inhibits the Activation of the Palmitic Acid-Induced TLR4/NF-ĸB Signaling Pathway in THP-1 Macrophages.
- Authors
Liu, S. P.; Li, X. Y.; Li, Z.; He, L. N.; Xiao, Y.; Yan, K.; Zhou, Z. G.
- Abstract
To investigate the effect of acylated ghrelin on the activation of TLR4/NF-ĸB signaling pathway induced by palmitic acid in human monocyte-derived (THP-1) macrophages, THP-1 macrophages were cultured for 12 h by palmitic acid with various concentrations. The THP-1 macrophages was pretreated by acylated ghrelin at different doses for 4 h before cultivated by palmitic acid (200 µmol/L) for 12 h. We observed the level of TLR4, NF-?B p65 phosphorylation in THP-1 macrophages and TNF-a, IL- 1ß in culture supernatant. TLR4 mRNA was measured by real-time PCR. TLR4 protein and NF-?B p65 phosphorylation was measured by western blotting. The expression of TNF-a and IL-1ß was detected by ELISA. Compared to the THP-1 macrophages without palmitic acid, the level of TLR4 mRNA protein and NF-ĸB p65 phosphorylation and the expression of TNF-a and IL- 1ß increased after treatment by palmitic acid in a dose-dependent fashion (P < 0.05). Compared to the THP-1 macrophages with palmitic acid (200 µmol/L), the level of the pervious substances decreased after preadministration by acylated ghrelin in a dose-dependent fashion. So, we make a conclusion that acylated ghrelin can regulate the activation of TLR4/NF-?B signaling pathway and inhibit the release of inflammatory cytokines in THP-1 macrophages which are stimulated by palmitic acid in a dose-dependent fashion.
- Subjects
MACROPHAGES; TYPE 2 diabetes risk factors; INFLAMMATION; INSULIN resistance risk factors; OBESITY complications; ANALYSIS of variance; CELLULAR signal transduction; POLYMERASE chain reaction; RESEARCH funding; WESTERN immunoblotting; GHRELIN; EQUIPMENT &; supplies; REVERSE transcriptase polymerase chain reaction; PHYSIOLOGY
- Publication
ISRN Endocrinology, 2012, p1
- ISSN
2090-4630
- Publication type
Article
- DOI
10.5402/2012/237613