We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Emptying of Intracellular Calcium Pool and Oxidative Stress Imbalance Are Associated with the Glyphosate-Induced Proliferation in Human Skin Keratinocytes HaCaT Cells.
- Authors
George, Jasmine; Shukla, Yogeshwer
- Abstract
We demonstrated that glyphosate possesses tumor promoting potential inmouse skin carcinogenesis and SOD1, calcyclin (S100A6), and calgranulin B (S100A9) have been associated with this potential, although the mechanism is unclear. We aimed to clarify whether imbalance in between [Ca2+]i levels and oxidative stress is associated with glyphosate-induced proliferation in human keratinocytes HaCaT cells. The [Ca2+]i levels, ROS generation, and expressions of G1/S cyclins, IP3R1, S100A6, S100A9, and SOD 1, and apoptosis-related proteins were investigated upon glyphosate exposure in HaCaT cells. Glyphosate (0.1mM) significantly induced proliferation, decreases [Ca2+]i, and increases ROS generation in HaCaT cells, whereas antioxidant N-acetyl-L-cysteine (NAC) pretreatment reverts these effects which directly indicated that glyphosate induced cell proliferation by lowering [Ca2+]i levels via ROS generation. Glyphosate also enhanced the expression of G1/S cyclins associated with a sharp decrease in G0/G1 and a corresponding increase in S-phases. Additionally, glyphosate also triggers S100A6/S100A9 expression and decreases IP3R1 and SOD 1 expressions in HaCaT cells. Notably, decreases Ca2+suppression also prevented apoptotic related events including Bax/Bcl-2 ratio and caspases activation.This study highlights that glyphosate promotes proliferation in HaCaT cells probably by disrupting the balance in between [Ca2+]i levels and oxidative stress which in turn facilitated the downregulation of mitochondrial apoptotic signaling pathways.
- Subjects
CALCIUM; CELL culture; CELL physiology; DERMATOLOGY; FLUORESCENT antibody technique; GROWTH factors; HERBICIDES; IMMUNOBLOTTING; KERATINOCYTES; ORGANOPHOSPHORUS compounds; PROTEOMICS; OXIDATIVE stress
- Publication
ISRN Dermatology, 2013, p1
- ISSN
2090-4592
- Publication type
Article
- DOI
10.1155/2013/825180