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- Title
Estudio de las condiciones de mezclado en fermentador para la producción de blastosporas de Beauveria bassiana.
- Authors
García-Gutiérrez, Cipriano; Berenice González-Maldonado, María; Medrano-Roldán, Hiram; Solís-Soto, Aquiles
- Abstract
In this work three fermenters were characterized: New Brunswick M-19 of 14 liters, Biocontroller Applikon 1035 of 7 liters and New Brunswick Bioflo III of 7 liters, determining the volumetric coefficient o×ygen transfer (KLa), gas hold up (GH) and the mi×ing time (tM . The fermenter New Brunswick Bioflo III had the best values with a ratio of diameter of the impeller/vessel (DI /DT ) of 0.43, KLa = 9.5-208 h-1 and tM = 1.0-3.0 s, so it was selected for the production of blastospores of Beauveria bassiana, using molasses as carbon source. We studied the mi×ing conditions, using different combinations of impellers, type Rushton, Lightnin and Ma×flo under a factorial e×perimental design 32 . The propagation time was of 4 days, working volume 4l, 10% inoculum (1×106 blastospores/ml), temperature 30°C, agitation of 400-500 rpm, aeration 0.5-1.0 vvm, and pH of 5.4. The fungus growth better using a combination of impellers Rushton-Ma×flo at 400 rpm and 1.0 vvm (F = 10.324, p ⩽ 0.0123) (LSD = 0.585) obtaining a concentration of 1.2×109 blastospores/ml, 2.2 g/l biomass and 2.48 g/l of substrate consumption (Y ×/s = 0.89). The mi×ing conditions and the parameters obtained can be applied to optimize the blastospore productions of B. bassiana to fermenter level in the e×perimental production of bioinsecticides.
- Subjects
BIOREACTORS; BEAUVERIA bassiana; BEAUVERIA; MOLASSES; BIOLOGICAL insecticides; BIOTECHNOLOGY
- Publication
Revista Colombiana de Biotecnología, 2013, Vol 15, Issue 2, p47
- ISSN
0123-3475
- Publication type
Article