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- Title
The study of GSDMB in pathogenesis of psoriasis vulgaris.
- Authors
Ji, Xiaojuan; Chen, Huaqing; Xie, Ling; Chen, Shiqi; Huang, Shan; Tan, Qi; Yang, Huifang; Yang, Tao; Ye, Xiaoying; Zeng, Zhaolin; Wan, Chunlei; Li, Longnian
- Abstract
Background: Gasdermin (GSDM) B is a member of the GSDM family, which is a protein that may be involved in the cell pyroptosis process and is associated with inflammatory diseases. Objective: To explore the correlation between GSDMB and psoriasis vulgaris. Methods: Skin lesions from 33 patients with psoriasis vulgaris and 69 normal controls were collected. ELISA and Western blot were adopted to detect proteins. The HaCaT cell line was transfected with 3 sets of interfering sequence siRNA, and the mRNA and protein levels before and after the transfection were measured by qPCR and Western blot respectively, so as to establish a cell model with low GSDMB gene expression; the MTT method was used to detect cells viability, flow cytometry to detect cell apoptosis. Results: The level of GSDMB protein in the skin lesions of patients with psoriasis vulgaris was lower than that in normal skin tissues (P < 0.05). The mRNA and protein expression levels of the target gene in the siRNA-GSDMB-3 group were lower than those in the control group (P < 0.05). The proliferation of HaCaT cells was decreased by MTT method and flow cytometry, and the apoptosis rate was increased (P < 0.05). Conclusion: The expression level of GSDMB in psoriasis vulgaris lesion tissue is lower than that of normal skin tissue. The down-regulation of GSDMB expression can inhibit cell proliferation and promote cell apoptosis. GSDMB may play a role in the pathogenesis of psoriasis by affecting the differentiation of keratinocytes and the function of T cells.
- Subjects
PSORIASIS; KERATINOCYTE differentiation; SKIN proteins; INHIBITION of cellular proliferation; GENE expression
- Publication
PLoS ONE, 2023, Vol 17, Issue 1, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0279908