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- Title
Effect of miR-301a/PTEN pathway on the proliferation and apoptosis of cervical cancer.
- Authors
Peng, Li-na; Shi, Wen-tian; Feng, Huan-rong; Wei, Chuan-yu; Yin, Qi-nan
- Abstract
The aim of this study was to evaluate the effect of the miR-301a/PTEN pathway in cervical cancer. miR-301a and PTEN expression were measured by quantitative real-time PCR (qRT-PCR) in tissues samples and HeLa cells. PTEN protein level was determined by Western blotting. Dual reporter luciferase assay was performed to validate PTEN as a direct target of miR-301a. The gain- and loss-of function assay was performed by miR-301a overexpression and silencing. Cell proliferation was monitored by cell counting Kit-8 (CCK-8). Cell apoptosis was quantitated by flow cytometry. SPSS was used to analyze the significant difference in the treatments. miR-301a demonstrated a significantly higher expression in cervical carcinoma tissues compared with the paired non-carcinoma tissues (n = 12), while PTEN expression was found to be significantly lower in cervical carcinoma tissues than their paired non-carcinoma tissues (n = 12). In addition, PTEN was identified as the direct target of miR-301a. Moreover, overexpression of miR-301a significantly promoted HeLa cells proliferation and anti-apoptosis which had a reverse pattern after PTEN overexpression. Our results confirm PTEN as a direct target of miR-301a in HeLa cells and suggest that miR-301a/PTEN pathway contributes to the development and progression of cervical cancer.
- Subjects
CERVICAL cancer; PTEN protein; HELA cells; APOPTOSIS; WESTERN immunoblotting
- Publication
Innate Immunity, 2019, Vol 25, Issue 4, p217
- ISSN
1753-4259
- Publication type
Article
- DOI
10.1177/1753425919840702