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- Title
The trends of peripheral blood leucocyte counts and MMP-9/ TIMP-1 ratio in acute experimental subarachnoid hemorrhage model in rats and their relationship with early brain injury.
- Authors
LI Dan-dong; SONG Jin-ning; AN Ji-yang; ZHANG Ming; SUN Peng; PANG Hong-gang; ZHAO Yong-lin; CHENG Mao-feng
- Abstract
Objective To investigate the trends of peripheral blood leucocyte counts and MMP-9/TIMP-1 ratio in acute experimental subarachnoid hemorrhage model in rats and their relationship wíth early brain injury. Methods A rat single cisterna magna injection model was used. Leukocytes were counted by blood counting chamber, and MMP-9 and TIMP-1 were measured by ELISA. The dry-wet weight method was used to measure brain water content. The inside diameter was measured on an HE stained basilar artery. Results The brain water content increased significantly at 6 h after subarachnoid hemorrhage and peaked at 24 h, so did the leukocyte count as well as MMP-9 and TIMP-1 contents. Both the leukocyte count and MMP-9/TIMP-1 ratio were related to early brain injury after subarachnoid hemorrhage. Conclusion The leukocyte count and MMP-9/TIMP-1 ratio increase significantly in the early stage of subarachnoid hemorrhage and return to normal after 72 h. The trend is related to early brain injury after subarachnoid hemorrhage. The MMP-9/TIMP-1 ratio may be used as a predictor of the appearance of early brain injury.
- Subjects
LEUCOCYTES; MATRIX metalloproteinases; SUBARACHNOID hemorrhage; LABORATORY rats; BRAIN injuries; BLOOD testing
- Publication
Journal of Xi'an Jiaotong University (Medical Sciences), 2013, Vol 34, Issue 6, p717
- ISSN
1671-8259
- Publication type
Article
- DOI
10.7652/jdyxb201306004