We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Selective propagation of mouse-passaged scrapie prions with long incubation period from a mixed prion population using GT1-7 cells.
- Authors
Miyazawa, Kohtaro; Masujin, Kentaro; Okada, Hiroyuki; Matsuura, Yuichi; Ushiki-Kaku, Yuko; Yokoyama, Takashi
- Abstract
In our previous study, we demonstrated the propagation of mouse-passaged scrapie isolates with long incubation periods (L-type) derived from natural Japanese sheep scrapie cases in murine hypothalamic GT1-7 cells, along with disease-associated prion protein (PrPSc) accumulation. We here analyzed the susceptibility of GT1-7 cells to scrapie prions by exposure to infected mouse brains at different passages, following interspecies transmission. Wild-type mice challenged with a natural sheep scrapie case (Kanagawa) exhibited heterogeneity of transmitted scrapie prions in early passages, and this mixed population converged upon one with a short incubation period (S-type) following subsequent passages. However, when GT1-7 cells were challenged with these heterologous samples, L-type prions became dominant. This study demonstrated that the susceptibility of GT1-7 cells to L-type prions was at least 105 times higher than that to S-type prions and that L-type prion-specific biological characteristics remained unchanged after serial passages in GT1-7 cells. This suggests that a GT1-7 cell culture model would be more useful for the economical and stable amplification of L-type prions at the laboratory level. Furthermore, this cell culture model might be used to selectively propagate L-type scrapie prions from a mixed prion population.
- Subjects
PRION diseases; PRION disease diagnosis; PRION disease prevention; TRANSMISSION of prion diseases; PRION disease treatment
- Publication
PLoS ONE, 2017, Vol 12, Issue 6, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0179317