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- Title
Effect of Prokaryotic Expressed Nucleoplasmin on the Efficiency of Somatic Cell Nuclear Transfer in Banna Mini-pig Inbred Line.
- Authors
Wenmin Cheng; Weirong Pan; Yubo Qing; Yingchao Liu; Xingqin Zha; Yan Huang; Jige Xin; Hongjiang Wei; Yangzhi Zeng
- Abstract
Nucleoplasmin (Npm) is the most abundant protein in the nucleus of oocytes which can promote in vitro nucleosome formation. In this study, to improve the efficiency of SCNT in Banna Mini-pig Inbred Line (BMI), we obtained the recombinant nucleoplasmin (Npm) by using a prokaryotic expression system and compared the difference on the developmental effects between exogenous Npm and its structural analog, polyglutamic acid (PGA). We chose the pMD18-T vector for Npm expression and transformed E. coli BL21 (DE3) cells to construct a prokaryotic expression system. The results showed that the recombinant Npm protein of 32 kDa could be obtained. Recombinant Npm or PGA was injected into porcine oocytes at different concentrations. The results showed that the blastocyst rates in injection 560 ng/μL Npm and 1000 ng/μL PGA groups were higher than those of other groups. Moreover, the blastocyst rate was highest in injection 560 ng/μL Npm group and was lowest in injection 100 ng/μL PGA group. These data suggested that addition of recombinant Npm obtained by prokaryotic expression or PGA could improve the development of SCNT embryos in the BMI.
- Subjects
NUCLEOPLASMIN; POLYGLUTAMIC acid; SOMATIC cell nuclear transfer; BLASTOCYST; PROKARYOTIC genomes; EMBRYOS
- Publication
Pakistan Journal of Zoology, 2017, Vol 49, Issue 1, p297
- ISSN
0030-9923
- Publication type
Article
- DOI
10.17582/journal.pjz/2017.49.1.297.303