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- Title
Adaptation of WM-68 Hybridoma Cell-line in Minimal Serum and Serum Free Culture Conditions.
- Authors
Hashmi, Furqan Khurshid; Cail, Russell; Islam, Muhammad; Saleem, Zikria; Amin, Umair; Hussain, Khalid; Saeed, Hamid
- Abstract
WM-68 cell line has been shown to produce anti-CD48 antibody - highly specific to B cell malignancies. For their clinical usage, cultivation of WM68 cells in animal serum pose a technical and safety disadvantage. Therefore, the present study was aimed to establish an adaptation protocol for WM68 hybridoma cell line - from high to a low serum concentration and finally into a serum free culture condition. The WM68 hybridoma cell line cultured in 10% FBS exhibited excellent cell culture profiles - cell viability, density and antibody production. Similar trend was observed when WM68 cell line was cultured in 0.5% FBS. However, in 0.5% FBS, data revealed poor antibody production in the stationary phase compared to 10% FBS. Finally, WM68 cell line cultured in 0.5% FBS, was passaged and plated in a serum free condition. Unfortunately, cells didn't survive past day one and manifested obvious signs of apoptosis. Taken together, these data suggested that 10% FBS and 0.5% FBS are suitable culture conditions for the propagation of WM68 hybridoma cell line and to generate antibodies. Thus, employing additional media factors and avant-garde cell culture techniques can enhance our understanding of the WM68 cell line culture requirements in a serum free media.
- Subjects
CELL lines; SERUM-free culture media; HYBRIDOMAS; B cell lymphoma; ANTIBODY formation; CELL survival
- Publication
Pakistan Journal of Zoology, 2014, Vol 46, Issue 2, p355
- ISSN
0030-9923
- Publication type
Article