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- Title
Fully automated macromolecule suppressed single voxel glutamate spectroscopy (FAMOUS SVGS).
- Authors
Reddy Nanga, Ravi Prakash; Hariharan, Hari; Reddy, Ravinder; Nanga, Ravi Prakash Reddy
- Abstract
<bold>Purpose: </bold>The aim of the study was to develop and validate a new localized (1)H MRS pulse sequence and automated post-processing software for the quantification of brain Glutamate (Glu) in clinical conditions at 7.0T in order to get reliable and reproducible results for acute intervention studies.<bold>Methods: </bold>Here we describe a new localized proton MRS method "Fully Automated MacrOmolecUle Suppressed Single Voxel Glutamate Spectroscopy (FAMOUS SVGS)" for measuring Glu. FAMOUS SVGS method consists of a new pulse sequence with optimized switchable water, metabolites and outer volume suppression modules, as well as a frequency selective inversion pulse and automated post-processing of the five spectra obtained. FAMOUS SVGS method was first validated with glutamate phantoms and then validated with test-retest repeatability studies in the occipital cortex of five normal volunteers at 7.0T.<bold>Results: </bold>Glutamate concentrations estimated from phantoms with FAMOUS SVGS method correlated well with actual concentrations. Test-retest repeatability studies in human brain in vivo yielded less than 0.3 mM intra-subject variations in Glu concentrations.<bold>Conclusions: </bold>FAMOUS SVGS method enables Glu quantification in vivo at 7.0T with test-retest variability of less than 0.3 mM. We expect that we can reliably measure ≥0.5 mM change in glutamate due to any acute intervention.
- Subjects
MACROMOLECULES; GLUTAMIC acid; SPECTROMETRY; IMAGING phantoms; STATISTICAL reliability; GLUTAMIC acid metabolism; AUTOMATION; COMPUTER software; MOLECULAR structure; NUCLEAR magnetic resonance spectroscopy; RESEARCH funding; WATER
- Publication
Journal of Translational Medicine, 2016, Vol 14, p1
- ISSN
1479-5876
- Publication type
journal article
- DOI
10.1186/s12967-016-0970-1