We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Degradation of oxalic acid by Trichoderma afroharzianum and its correlation with cell wall degrading enzymes in antagonizing Botrytis cinerea.
- Authors
Wu, Xiaoqing; Lyu, Yuping; Ren, He; Zhou, Fangyuan; Zhang, Xinjian; Zhao, Xiaoyan; Zhang, Guangzhi; Yang, Hetong
- Abstract
Aim: Oxalic acid (OA) is one of the pathogenic factors of Botrytis cinerea. Trichoderma afroharzianum exerts both antagonistic and oxalate‐degrading effects on B. cinerea. This study aimed to investigate the relationship between the elimination of OA by T. afroharzianum and its antagonistic effects on B. cinerea. Methods and Results: Reversed‐phase high performance liquid chromatogram (RP‐HPLC) analysis showed that T. afroharzianum LTR‐2 eliminated 10‐ or 20‐mmol/L OA within 120 h, with the degradation being particularly efficient at the concentration of 20 mmol/L. RNA‐seq analysis showed that the oxalate decarboxylase (OXDC) gene Toxdc, β‐1,3‐exoglucanase gene Tglu and aspartic protease gene Tpro of LTR‐2 were significantly upregulated after treatment with 20‐mmol/L OA. RT‐qPCR analysis showed that under the conditions of confrontation, Toxdc and three cell wall degrading enzyme (CWDE) genes were upregulated before physical contact with B. cinerea. In addition, RT‐qPCR analysis showed that OA synthesis in B. cinerea was not significantly affected by LTR‐2. Conclusions: The results revealed a correlation between OA degradation and mycoparasitism in T. afroharzianum when antagonising B. cinerea at the transcriptional level. Significance and Impact of the Study: The relationship between OA degradation by T. afroharzianum and its effects against B. cinerea provide a new perspective on the antagonism of T. afroharzianum against B. cinerea. In addition, this study provides theoretical data for the scientific application of T. afroharzianum in the field of biocontrol.
- Subjects
OXALIC acid; BOTRYTIS cinerea; TRICHODERMA; BACTERIAL cell walls; OXALATES; ENZYMES
- Publication
Journal of Applied Microbiology, 2022, Vol 133, Issue 5, p2680
- ISSN
1364-5072
- Publication type
Article
- DOI
10.1111/jam.15617