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- Title
Detection and characterization of a bacteriocin, garviecin L1-5, produced by Lactococcus garvieae isolated from raw cow’s milk.
- Authors
Villani, F.; Aponte, M.; Blaiotta, G.; Mauriello, G.; Pepe, O.; Moschetti, G.
- Abstract
Aims: The identification of a bacteriocin-producing lactococcal strain isolated from raw cow’s milk is reported, along with production conditions, physical and chemical properties, and mode of action of the bacteriocin. Methods and Results: On the basis of resistance to clindamycin, species-specific PCR and amplification of the 16S-23S rDNA spacer region, the strain was identified as Lactococcus garvieae. Its bacteriocin, designated garviecin L1-5, was bactericidal against closely related species and strains of species from different genera, including Listeria monocytogenes and Clostridium spp. Garviecin L1-5 was shown to be proteinaceous by protease inactivation and was unaffected by heat treatments, also at low pH values. When amplifying known lactococcal bacteriocin genes using DNA from strain L1-5 as template, no amplification products were observed on the agarose gel. The molecular weight of garviecin L1-5 was about 2·5 kDa. As far as is known, no bacteriocins have been detected from Lactococcus garvieae. Conclusions: The general properties of garviecin L1-5 are characteristic of the low-molecular-weight bactericidal peptide group. Significance and Impact of the Study: The survey of micro-organisms for novel antimicrobial substances provided valuable information on their physiology, ecology and practical application.
- Subjects
LACTOCOCCUS; RECOMBINANT DNA
- Publication
Journal of Applied Microbiology, 2001, Vol 90, Issue 3, p430
- ISSN
1364-5072
- Publication type
Article
- DOI
10.1046/j.1365-2672.2001.01261.x