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- Title
Modulation of IgD and CD20 by Ligation of CD5 on Tonsillar B Cells.
- Authors
Morikawa, K; Torii, I; Morikawa, S
- Abstract
The CD5 molecule, pan T cell marker, has been known to be expressed on a minor population of B cells, termed B-1 cells. However, the physiological function and pathological role of CD5+ B (B-1) cells remain to be fully elucidated in humans. In the present study, we aimed to clarify the significance of CD5 expression on the B lymphocytes in human tonsil. Using flow cytometric analysis by three-colour immunofluorescence staining, we observed a majority of the cell surface CD5-positive (sCD5+ ) B cells among the sIgD+ B-cell population, as previously described. Contrary to our expectation, approximately half of the sIgD+ /sCD5+ B cells expressed CD38 on their cell surface. Furthermore, a small number of sCD5+ were observed in the sIgD- B cell population. The addition of anti-CD5 monoclonal antibody (MoAb) to the culture induced downmodulation of sCD20 and sIgD of the tonsillar B cells, resulting in an increase of sCD38- /sIgD- (memory) B cells during the 10 day culture periods in the CD40/l cell culture system. Our findings suggest that ligation of CD5 might transduce the signal to regulate B cell maturation.
- Subjects
B cells; TONSILS; CD antigens; CYTOLOGY
- Publication
Scandinavian Journal of Immunology, 2002, Vol 55, Issue 1, p44
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1046/j.1365-3083.2002.00987.x