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- Title
Oxidative Burst in Human Epstein--Barr Virus--Transformed B--Cell Lines Triggered by Immobilized Specific Antigen.
- Authors
Maly, F.-E.; Urwyler, A.; Tengler, R.; Steinitz, M.; Golding, B.; De Weck, A. L.
- Abstract
Normal human B lymphocytes and Epstein-Barr virus-transformed B-cell lines can produce reactive oxygen species such as superoxide if treated with phorbol myristate acetate (PMA) or with the surface immunoglobulin cross-linking agents protein A and anti-immunoglobulin. Here, we investigated under which conditions specific antigen, the natural legend of surface immunoglobulin, can stimulate an oxidative burst in monoclonal Epstein Barr virus-transformed B-cell lines producing antibodies of known specificities. After a short lag time of 1-2 min, exposure to the specific antigen stimulated a prolonged oxidative burst (tmax 30-90 min), as measured by Lucigenin-enhanced, superoxide dismutase-inhibitable chemiluminescence in the corresponding line only. The effect was induced in each line if the specific antigen was immobilized lo a solid support. Except in one line in which antigen also stimulated an oxidative burst if presented at relatively high density on a soluble carrier, soluble antigen did not induce B-cell oxidase activation. This suggests that normal, non-transformed B lymphocytes also require interaction with relatively dense deposits of specific antigen for activation of their oxidase.
- Subjects
LYMPHOCYTES; B cells; REACTIVE oxygen species; SUPEROXIDE dismutase; CELL culture; CELL lines
- Publication
Scandinavian Journal of Immunology, 1990, Vol 32, Issue 6, p661
- ISSN
0300-9475
- Publication type
Article
- DOI
10.1111/j.1365-3083.1990.tb03208.x