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- Title
A review of conventional detection and enumeration methods for pathogenic bacteria in food.
- Authors
Gracias, Kiev S.; McKillip, John L.
- Abstract
With continued development of novel molecular-based technologies for rapid, high-throughput detection of foodborne pathogenic bacteria, the future of conventional microbiological methods such as viable cell enumeration, selective isolation of bacteria on commercial media, and immunoassays seems tenuous. In fact, a number of unique approaches and variations on existing techniques are currently on the market or are being implemented that offer ease of use, reliability, and low cost compared with molecular tools. Approaches that enhance recovery of sublethally injured bacteria, differentiation among species using fluorogenics or chromogenics, dry plate culturing, differentiation among bacteria of interest using biochemical profiling, enumeration using impedence technology, techniques to confirm the presence of target pathogens using immunological methods, and bioluminescence applications for hygiene monitoring are summarized here and discussed in relation to their specific advantages or disadvantages when implemented in a food microbiology setting.
- Subjects
PATHOGENIC bacteria; BACTERIAL diseases; PATHOGENIC microorganisms; FOOD handling; FOOD safety; LUMINESCENCE; BIOLUMINESCENCE
- Publication
Canadian Journal of Microbiology, 2004, Vol 50, Issue 11, p883
- ISSN
0008-4166
- Publication type
Article
- DOI
10.1139/W04-080