We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Effects of Homologous Expression of 1,4-Benzoquinone Reductase and Homogentisate 1,2-Dioxygenase Genes on Wood Decay in Hyper-Lignin-Degrading Fungus Phanerochaete sordida YK-624.
- Authors
Mori, Toshio; Koyama, Genki; Kawagishi, Hirokazu; Hirai, Hirofumi
- Abstract
We investigated the function of 1,4-benzoquinone reductase (BQR)- and homogentisate 1,2-dioxygenase (HGD)-like genes in wood degradation by Phanerochaete sordida YK-624, which exhibits high ligninolytic activity and selectivity. We determined homologous expression in the genomic and cDNA sequences of BQR- and HGD-like genes in P. sordida YK-624 ( PsBQR and PsHGD). Both genes shared high homology (≥90 % amino acid sequence similarity) with the corresponding genes in Phanerochaete chrysosporium. These genes were co-transformed with a reporter gene into an uracil auxotrophic mutant of P. sordida YK-624. The PsBQR and PsHGD co-transformants exhibited lower holocellulolytic activity and higher ligninolytic selectivity than the control transformants. In liquid culture with vanillin, both co-transformants significantly accelerated vanillin degradation. Thus, we suggest that the rapid metabolism of low-molecular weight lignin fragments, due to the homologous expression of BQR- and HGD-like genes, affects quinone redox cycling to produce hydroxyl radicals, thereby decreasing holocellulose degradation and increasing ligninolytic selectivity.
- Subjects
BENZOQUINONES; DIOXYGENASES; MOLECULAR genetics; NUCLEOTIDE sequencing; AMINO acid sequence; VANILLIN
- Publication
Current Microbiology, 2016, Vol 73, Issue 4, p512
- ISSN
0343-8651
- Publication type
Article
- DOI
10.1007/s00284-016-1089-6