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- Title
New method for quantification of Trypanosoma cruzi in animal’s tissue in the chronic phase of experimental Chagas' disease.
- Authors
Esperandim, Viviane Rodrigues; Ferreira, Daniele da Silva; Alonso Toldo, Miriam Paula; Saraiva, Juliana; Augusto, Mariana Bryan; de Albuquerque, Sérgio
- Abstract
We developed a new method for the quantification of parasites in tissue. Trypanosoma cruzi strain CL parasites were genetically engineered to express the Escherichia coli β-galactosidase gene, lacZ and this enzyme is able to catalyze a colorimetric reaction with chlorophenol red β- d galactopyranoside (CPRG) as the substrate. The animals were infected with clone CL Brener strain B5 of T. cruzi and treated with benznidazole in order to verify the reduction in the number of parasites in tissue study by quantifying the enzyme β-galactosidase. The assay demonstrates a reduction in the number of parasites in the groups treated. Thus, this test can be used to test other substances with the aim of verifying the effectiveness in the chronic phase of experimental Chagas' disease.
- Subjects
TRYPANOSOMA cruzi; CHAGAS' disease; RECOMBINANT microorganisms; CHLOROPHENOLS; ANIMAL diseases; TISSUES
- Publication
Parasitology Research, 2010, Vol 106, Issue 6, p1471
- ISSN
0932-0113
- Publication type
Article
- DOI
10.1007/s00436-010-1780-7