We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
The levels of peroxisomal catalase protein and activity modulate the onset of cell death in tobacco BY-2 cells via reactive oxygen species levels and autophagy.
- Authors
Tyutereva, Elena V.; Dobryakova, Ksenia S.; Schiermeyer, Andreas; Shishova, Maria F.; Pawlowski, Katharina; Demidchik, Vadim; Reumann, Sigrun; Voitsekhovskaja, Olga V.
- Abstract
In plant cells, peroxisomes participate in the metabolism of reactive oxygen species (ROS). One of the major regulators of cellular ROS levels – catalase (CAT) – occurs exclusively in peroxisomes. CAT activity is required for immunity-triggered autophagic programmed cell death (PCD). Autophagy has been recently demonstrated to represent a route for degradation of peroxisomes in plant cells. In the present study, the dynamics of the cellular peroxisome pool in tobacco BY-2 cell suspension cultures were used to analyse the effects of inhibition of basal autophagy with special attention to CAT activity. Numbers of peroxisomes per cell, levels of CAT protein and activity, cell viability, ROS levels and expression levels of genes encoding components of antioxidant system were analysed upon application of 3-methyladenine (3-MA), an inhibitor of autophagy, and/or aminotriazole (AT), an inhibitor of CAT. When applied separately, 3-MA and AT led to an increase in cell death, but this effect was attenuated by their simultaneous application. The obtained data suggest that both the levels of CAT protein in peroxisomes as well as CAT activity modulate the onset of cell death in tobacco BY-2 cells via ROS levels and autophagy.
- Subjects
CATALASE; CELL death; REACTIVE oxygen species; PLANT cells &; tissues; PLANT metabolism; PEROXISOMES; PLANTS
- Publication
Functional Plant Biology, 2018, Vol 45, Issue 1/2, p247
- ISSN
1445-4408
- Publication type
Article
- DOI
10.1071/FP16418