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- Title
Peimisine ameliorates DSS-induced colitis by suppressing Jak–Stat activation and alleviating gut microbiota dysbiosis in mice.
- Authors
Li, Yue; Yang, Xia; Han, Jicheng; Bai, Bing; Li, Yaru; Shang, Chao; Li, Shanzhi; Xiu, Zhiru; Liu, Zirui; Ge, Chenchen; Zhu, Guangze; Jin, Ningyi; Fang, Jinbo; Li, Yiquan; Li, Xiao; Zhu, Yilong
- Abstract
Objectives: Inflammatory cytokine secretion and gut microbiota dysbiosis play crucial roles in ulcerative colitis. In this research, the protective effects of peimisine on colitis mice were investigated. Methods: The protective effects were evaluated by the disease activity index, colonic length, hematoxylin–eosin, and AB/PAS Staining. The protective mechanisms were analyzed by ELISA, Western-blot, immunohistochemistry staining, immunofluorescence staining, and 16S rRNA gene analysis. Key findings: The results showed that peimisine treatment could reduce the disease activity index, prevent colonic shortening, and alleviate colon tissue damage. Peimisine treatment also decreased the levels of MCP-1, IL-1β, IL-6, IFN-γ, TNF-α and affected macrophage polarization and Th17/Treg cell balance by downregulating the expression of jak1/2, p-jak1/2, stat1/3, and p-stat1/3. Moreover, peimisine treatment significantly increased the abundances of beneficial microbes (e.g. Ruminococcaceae UCG-014 and Lachnospiraceae_NK4A136_group) and decreased the abundances of harmful microbes (e.g. Bacteroides and Escherichia). Conclusions: Peimisine can ameliorate colitis by inhibiting Jak–Stat signaling pathway, reversing gut microbiota alterations, suppressing macrophage M1 polarization, maintaining the Th17/Treg cell balance, and reducing sustained inflammatory cytokines-related inflammatory injury.
- Subjects
GUT microbiome; COLITIS; JAK-STAT pathway; DYSBIOSIS; ULCERATIVE colitis
- Publication
Journal of Pharmacy & Pharmacology, 2024, Vol 76, Issue 5, p545
- ISSN
0022-3573
- Publication type
Article
- DOI
10.1093/jpp/rgad091